This enzyme allows amplification of simple and complex dna templates over a large range of target sizes and provides 6x higher fidelity over taq. Taq dna polymerase is heatstable and will synthesize dna at elevated temperatur es from singlestranded templates in the presence of a primer. Efficiency comparison of seven different taq polymerases used. The superscript iii onestep rtpcr system with platinum taq dna polymerase is designed for the sensitive, reproducible, endpoint detection and analysis of rna molecules by rtpcr. Links to pubmed are also available for selected references. Get a printable copy pdf file of the complete article 394k, or click on a page image below to browse page by page. The enzyme consists of a single polypeptide with a molecular weight of 94 kda. Taq dna polymerase fast and robust pcr neb is proud to taq dna polymerase neb is proud to represent taq dna polymerase on the basis that it is an enzyme of the highest quality and value. Platinum taq dna polymerase is a recombinant taq polymerase complexed with a proprietary antibody that blocks the polymerase activity at ambient temperatures. Day 1 start 3ml overnight culture of taq from glycerol stock in lbamp 75ngul day 2 add 1ml of overnight culture to 1l of lbamp.
Use platinum taq dna polymerase for the amplification of dna from complex genomic, viral, and plasmid templates, as well as in rtpcr. Safety data sheet safety data sheet according to ec directive 19072006ec r faststart taq dna polymerase, u version 2. Bioneers taq dna polymerase is isolated from recombinant. Best profiles from samples were given by taq dna invitrogen, amplitaq gold, jumpstart taq and gel form polymerases.
The invitrogen accuprime taq dna polymerase, high fidelity, provides qualified reagents for the highfidelity pcr amplification of nucleic acid templates. The enzyme is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. This enzyme has a 5 to 3 dna polymerase exonuclease activity but lacks a 3 to 5 exonuclease activity. Scientists realized that thermostable heatstable dna polymerases would be needed for pcr to work efficiently. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kda. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step. Taq dna polymerase is a thermostable dna polymerase that catalyzes the polymerization of nucleotides into duplex dna in the 5 3 direction.
Reagent kit size volume description itaq dna polymerase 250 u 50 l itaq dna polymerase, 5 ul clear cap 10x itaq buffer 1. Lost of alleles was observed in longest amplified fragments of sgm plus loci, d2s38. The streamlined, easytofollow protocol provided with the kit simplifies pcr setup. Using this convenient onestep formulation, you can perform both cdna synthesis and pcr amplification in a single tube using genespecific primers, and target. Oct 11, 1993 full text is available as a scanned copy of the original print version. Taq dna polymerase is a highly thermostable dna polymerase of the thermophilic bacterium thermus aquaticus. Both will leave a 3 da overhang on 30% of the ends of pcr fragments. Taq dna polymerase new england biolabs lost practically all of the alleles. Platinum taq dna polymerase thermo fisher scientific. It is supplied with 10x standard taq reaction buffer. Platinum hotstart technology inhibits dna polymerase activity at ambient temperatures, allowing room temperature. The polymerase chain reaction pcr method for amplifying selectively discrete segments of dna has found widespread applications in molecular biology, due in part, to the substitution of a thermostable dna polymerase isolated from thermus aquaticus taq 1 for the previously used e. Ensure successful pcr using nebs pcr protocol for taq dna polymerase with standard taq buffer m0273. Taq dna polymerase is the industry standard for routine pcr.
Invitrogen platinum taq dna polymerase is a convenient and reliable hot start thermostable dna polymerase for pcr that provides enhanced specificity over that of taq dna polymerase. Taq dna polymerase is an enzyme widely used in pcr 2. One unit of taq dna polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into dna in 30 minutes at 74c. The polymerase chain reaction pcr method for amplifying selectively discrete segments of dna has found widespread applications in molecular biology, due in part, to the substitution of a. Taq dna polymerase, recombinant thermo fisher scientific. Taq polymerase has substantial enzymatic activity at 37c, although its optimal activity is expressed at a much higher temperature approximately 72c. The polymerase chain reaction pcr was developed by chemist kary mullis in the 1980s, as a means to make many copies of dna fragments. Pluthero 1993 rapid purification of highactivity taq dna polymerase. Full text is available as a scanned copy of the original print version.
In general, they have maximal catalytic activity at 75 to 80. The enzyme is in a recombinant form, expressed in e. It includes an enzyme blend of recombinant taq dna polymerase, pyrococcus species gbd polymerase, and platinum taq antibody. Neb provides high quality recombinant taq at an exceptional value.
Superscript iii onestep rtpcr system with platinum taq. Genscript taq dna polymerases are highly thermostable recombinant dna polymerases and ideally suited for routine pcr reactions. Pcr protocol for taq dna polymerase with standard taq buffer m0273 protocols. Taq dna polymerase is isolated from thermus aquaticus yt1. It is supplied with 10x standard taq reaction buffer, which is detergentfree and designed to be compatible with existing assay systems. Accuprime taq dna polymerase, high fidelity, product. Use an additional ddh 2 o as negative control for the third exponential pcr. Invitrogen taq dna polymerase, recombinant nondistribution product as customer accommodation. This enzyme has a 5 to 3 dna polymerase exonuclease activity but lacks a 3 to 5 exonuclease. Taq dna polymerase is supplied with the unique qiagen pcr buffer that.
Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. The polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. Taq polymerase, being thermostable, proved ideal for pcr. Taq dna polymerase recombinant taq dna polymerase is the enzyme of choice for most pcr applications. Taq is available with different formats to accommodate a variety of pcr applications. The 5x green gotaq reaction buffer contains two dyes blue and yellow that separate during electrophoresis to monitor migration progress. Platinum superfi dna polymerase is a proofreading dna polymerase that combines fidelity with platinum hotstart technology, and is ideally suited for cloning, mutagenesis, and other applications. Platinum taq dna polymerase high fidelity contains recombinant taq dna polymerase, pyrococcus species gbd polymerase, and platinum taq antibody. Available on gsava contract for federal government. Taq dna polymerase is recombinant taq dna polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Taq dna polymerase is a thermostable enzyme that synthesizes dna from singlestranded templates in the presence of dntps and a primer. Taq dna polymerase outperformed kits tested from other suppliers and delivers robust pcr performance in a wide range of pcr conditions, without the need for timeconsuming optimization see figures tolerance of different primer t m values and specific amplification of long pcr products. For multiple reactions, prepare a master mix of components common to all reactions to minimize pipetting error, and then dispense appropriate volumes into each 0.
Taq dna polymerase is a thermostable dna polymerase that possesses a 5. The enzyme has terminal transferase activity which results in the addition of a single nucleotide adenosine at 3 end of the extension product. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus, from which it was originally isolated by chien et al. Taq dna polymerase outperformed kits tested from other suppliers and delivers robust pcr performance in a wide range of pcr conditions, without the need for timeconsuming optimization see figures. Kit size component 100 u 250 u 500 u 1500 u 5,000 u.
Onetaq dna polymerase is an optimized blend of taq and deep vent dna polymerases for use with routine and difficult pcr experiments. Rapid purification of highactivity taq dna polymerase. A protocol for the use of gotaq dna polymerase with 5x green gotaq reaction buffer and 5x colorless gotaq reaction buffer. Taq dna polymerase, recombinant protocol 20142taq dna polymerase protocol the example pcr procedure below shows appropriate volumes for a single 50l reaction. The following guidelines are provided to ensure successful pcr using nebs taq dna polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. The taq dna polymerase is the most successful application of a product derived from an extremophile, with annual sales around half a billion dollars podar and reysenbach, 2006. Purification of taq dna polymerase for 1 liter culture modified from the protocol presented in f. Taq dna polymerase is appropriate for use in the amplification of dna from complex genomic, viral, and plasmid templates, rtpcr, sequencing ssdna, and cycle sequencing unit definition. Invitrogen taq dna polymerase, recombinant 500 units.
It has a 53 dna polymerase activity and a 53 exonuclease activity. Using this convenient onestep formulation, you can perform both cdna synthesis and pcr amplification in a single tube using genespecific primers, and target rnas from either total rna or mrna. Taq dnapolymerase, from thermus aquaticus recombinant, expressed in escherichia coli catalog number d1806 storage temperature 20 c technical bulletin introduction taq dna polymerase is a thermostable enzyme derived from the thermophilic bacterium thermus aquaticus. Amplification of templates with high gc content, high. Efficiency comparison of seven different taq polymerases. Taq dna polymerase is appropriate for use in the amplification of dna from complex genomic, viral, and plasmid templates, rtpcr, sequencing ssdna, and cycle sequencing unit. Bioneers taq dna polymerase is isolated from recombinant li strain containing the dna polymerase gene from thermus aquaticus yt1. Mb000042eut0 is a thermostable dna polymerase purified from an e. Taq dna polymerase is heatstable and synthesizes dna at elevated temperatures from singlestranded templates in the presence of. Invitrogen sells the native form of the taq dna polymerase and a cloned version that is expressed in e. Taq dna polymerase can be used in simple, routine pcr.
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